Threads by latest replies - Page 2489

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No.13691008 ViewReplyOriginalReport
Is there any known link between autism and pedophilia? Or more specifically, autism and psychological trauma from child sexual abuse?
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No.13690824 ViewReplyOriginalReport
arguing with globetards be like
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covid vax science

No.13688115 ViewReplyLast 50OriginalReport
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No.13691315 ViewReplyOriginalReport
Who is the Anti Defamation League of Science?
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No.13688279 ViewReplyOriginalReport
Do not think about free energy
Do not experiment with free energy devices
Free energy is impossible
Ridicule and ignore free energy
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(22 replies)
No.13687658 ViewReplyOriginalReport
Why are women enrolling in college at higher rates than men?
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(5 replies)
No.13690196 ViewReplyOriginalReport
I am a finitist mainly because the most simple problems when you introduce infinities seem completely unsolvable and even meaningless (particularly CH but also many other problems) but this article by CH intrigued me.
https://www.ams.org/notices/200106/fea-woodin.pdf
In it he explains how second order arithmetic can also be almost as real as first-order arithmetic as long as you assume projective determinacy.
>The next structure, H(?1), is also a familiar one. It is essentially just the structure P(N), N, +, ·, ? , which is the standard structure for Second Order Number Theory. Of course, neither N, +, · nor P(N), N, +, ·, ? is a structure for the language L(ˆ=, ?ˆ), but each is naturally a structure for a specific formal language which is easily defined. There are natural questions about H(?1) which are not solvable from ZFC. However, there are axioms for H(?1) which resolve these questions, providing a theory as canonical as that of number theory, and which are clearly true. But the truth of these axioms became evident only after a great deal of work. For me, a remarkable aspect of this is that it demonstrates that the discovery of mathematical truth is not a purely formal endeavor.
I don't know bros, but if I read up on this and what he says is actually true, I might become a (second-order but no higher) infinitist. It would be a good thing because I love mathematics and most of it I cannot justify from my measly first-order arithmetic framework. The reverse mathematics program suddenly becomes very relevant again.
What does /sci/ think about this?
And where can I find a nice exposition on projective sets which would convince me of Woodin's thesis?
(46 replies)
No.13687540 ViewReplyOriginalReport
Vax Chads just can't stop winning.
Then again, it was always kind of weird that those predicting the vaxed dropping dead en masse in the next 2weekz/2yearz were at the same time preparing for their favorite vaxed political figure to mount a 2024 run.
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(36 replies)

COVID Test Accuracy Fight IS Not Over !!!

No.13680979 ViewReplyOriginalReport
*I am not a Medical Professional*

Human DNA has over 3,000,000,000 base pairs.
The PCR test makes a replica of that 3,000,000,000 base pairs every cycle.
Cycles - DNAs
13 4,096.00
14 8,192.00
15 16,384.00
16 32,768.00
17 65,536.00
18 131,072.00
19 262,144.00
20 524,288.00
21 1,048,576.00
22 2,097,152.00
23 4,194,304.00
24 8,388,608.00
25 16,777,216.00
26 33,554,432.00
27 67,108,864.00
28 134,217,728.00
29 268,435,456.00
30 536,870,912.00
31 1,073,741,824.00
32 2,147,483,648.00
33 4,294,967,296.00
34 8,589,934,592.00
35 17,179,869,184.00
36 34,359,738,368.00
37 68,719,476,736.00
38 137,438,953,472.00
*Note: at higher cycles, the amount of new DNA generated slows down from 2x the previous cycle, to eventually add no new DNA, because the excess material for replicating DNA in the cells (which the DNA came out of), has ran out.

The amount of Cycles you run is determined by timed runs.
The PCR tester cannot visually see the DNA multiplying, therefore, they use a chart and temperature to estimate the number of cyles that have actually occured.

Denaturation
Temp: 95°C. Time: 5 min on initial cycle; 30 seconds to 1 min on rest

Annealing
Temp: 5°C below Tm of primers; no lower than 40°C. Time: 30-45 seconds. This is the step where you would use a gradient.

Extension
Temp: 72°C. Time: ~1 min/kb of expected product; 5-10 min on last cycle.

Number of Cycles
~30 cycles

2012 video on PCR - youtu.be/4sXPUbIrh3A?t=44

Every cycle, there is a different chance for DNA mutation, which occurs at higher levels for different DNA samples.
If DNA in the body mutates, the cell it is in, is known as cancerous.
Different people with different DNA have different risks for DNA mutation.
Therefore, PCR accuracy is an aggregate measure of many different DNA samples.
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