It's a combination of Peter having massive guilt over Uncle Ben and the fact that he is, non-ironically, a fucking quip machine. His rogue's gallery are quite literally groomed to understand and respond to the constant stream of zingers and one-liners the web head throws at them because it's part of the game. They menace him, he does a few quips, and he (mostly) wins.
But then they make the mistake of threatening his friends or his family. Then the quips stop. Then the fun stops. Now you have a Spider-Man who is not only silent (Like what he did with Shrunken Bones or whatever the fuck that dude's name is), but you also have a Spider-Man who's willing to rip the face of Norman Osborn and Kraven's wife. The human part of both Superman and Spider-Man are their most terrifying character traits because when pushed too far, God knows what they're capable of and willing to do to you.