They use a RNA concentration of zero point two milligram per milliliter.
>https://www.ema.europa.eu/en/documents/assessment-report/covid-19-vaccine-moderna-epar-public-assessment-report_en.pdf
>In Phase I and II studies, a target concentration of 0.5 mg/mL mRNA was prepared and a range of doses tested. After selection of the final dose, a target concentration of 0.20 mg/mL mRNA was developed for Phase III and commercial batches as a ready-to-use solution.
Also, they use zero point 5 milliliter per shot
>https://www.fda.gov/media/144637/download
>The Moderna COVID-19 Vaccine is supplied in two multiple-dose vial presentations: oA multiple-dose vial containing a maximum of 11 doses: range 10-11 doses (0.5 mL each). oA multiple-dose vial containing a maximum of 15 doses: range 13-15 doses (0.5 mL each).
The Pfizer vaccine is 4282 base pairs
>https://blog.jonasneubert.com/2021/01/10/exploring-the-supply-chain-of-the-pfizer-biontech-and-moderna-covid-19-vaccines/
I guess it's similar for moderna. Let's say moderna is 5000 base pairs, because that's easier to calculate. 5000 bp is 1.6 million gram per mole. And if you use 1 microgram, then that's 62 picomoles = 3.75 x 10^13 molecules.
>https://www.thermofisher.com/de/de/home/references/ambion-tech-support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html
They take per shot half a milliliter with a concentration of 0.2 mg/ml, that's 0.1 milligram total. 100µg RNA is 3.75 x 10^15, that means 4000 trillions of mRNA molecules.
Is that calculation correct?
How many lipid vesicles do they inject? I didn't find a source for that.
How many mRNA molecules are inside of one lipid vesicle?
>https://www.ema.europa.eu/en/documents/assessment-report/covid-19-vaccine-moderna-epar-public-assessment-report_en.pdf
>In Phase I and II studies, a target concentration of 0.5 mg/mL mRNA was prepared and a range of doses tested. After selection of the final dose, a target concentration of 0.20 mg/mL mRNA was developed for Phase III and commercial batches as a ready-to-use solution.
Also, they use zero point 5 milliliter per shot
>https://www.fda.gov/media/144637/download
>The Moderna COVID-19 Vaccine is supplied in two multiple-dose vial presentations: oA multiple-dose vial containing a maximum of 11 doses: range 10-11 doses (0.5 mL each). oA multiple-dose vial containing a maximum of 15 doses: range 13-15 doses (0.5 mL each).
The Pfizer vaccine is 4282 base pairs
>https://blog.jonasneubert.com/2021/01/10/exploring-the-supply-chain-of-the-pfizer-biontech-and-moderna-covid-19-vaccines/
I guess it's similar for moderna. Let's say moderna is 5000 base pairs, because that's easier to calculate. 5000 bp is 1.6 million gram per mole. And if you use 1 microgram, then that's 62 picomoles = 3.75 x 10^13 molecules.
>https://www.thermofisher.com/de/de/home/references/ambion-tech-support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html
They take per shot half a milliliter with a concentration of 0.2 mg/ml, that's 0.1 milligram total. 100µg RNA is 3.75 x 10^15, that means 4000 trillions of mRNA molecules.
Is that calculation correct?
How many lipid vesicles do they inject? I didn't find a source for that.
How many mRNA molecules are inside of one lipid vesicle?
