>>12969414The authors did several things: they overexpressed LINE1 elements (retrotransposons) and showed results suggesting that cytokine exposure can cause some upregulation of things that might trigger the induction of reverse transcriptases.
Based just on that paper I'd suggest that active COVID infection be a contradindication for vaccination. My next question would be - how do the levels of cytokine treatment they used in the paper stack up against the levels of cytokine induced by vaccination, or against what you get in a severe COVID infection? They used conditioned media from several cell culture types in their cytokine experiments, so I have no idea how that compares to clinically relevant cytokine events.
If cytokine levels in the paper are far above what would be clinically relevant, either from vaccination or active infection, there might be very little to worry about. If it's close to or below what you get from clinically relevant events, there might be cause for concern.
They also noted that it wasn't entire COVID genomes that were being integrated, but a specific ORF (Sequence N, see figure 1) that happened to be close enough to what the reverse transcriptases were looked for. So my next question is, are there any sequences in the vaccine constructs that are similar to that ORF? If there are, again, might be cause for concern. If there aren't, probably not much to worry about.
I also am not familiar with human cell culture and immunology, so I cannot begin to assess their experiments or interpretations on scientific validity or informative value. I'm taking what they say at face value. I would appreciate the input of someone who DOES know the biological systems in question.
