/sci/ - Science & Math » Thread #12885900

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Anonymous Fri 26 Mar 15:18:53 2021 No.12885900 View Reply Original Report

Quoted By: >>12885909 >>12885965 >>12886163 >>12886170 >>12886192 >>12886215 >>12886326 >>12886472

So I created this organometallic solution and the shit creeped out overnight. Never had it happen this fast and I can watch it grow basically if I check every hour

Anonymous

Anonymous Fri 26 Mar 2021 15:20:55 No.12885909 Report

Quoted By:

>>12885900
Cap your cum jars op
Splooge remains active for a while after leaving the body and sometimes they can "crawl" out of uncapped containers

Anonymous

Anonymous Fri 26 Mar 2021 15:34:10 No.12885965 Report

Quoted By: >>12886122

>>12885900
Smoke it.

Anonymous

Anonymous Fri 26 Mar 2021 16:06:22 No.12886122 Report

Quoted By:

>>12885965
this kills the Smoker

Anonymous

Anonymous Fri 26 Mar 2021 16:14:30 No.12886163 Report

Quoted By: >>12886222

>>12885900
Pretty cool
What's in it?

Anonymous

Anonymous Fri 26 Mar 2021 16:15:56 No.12886170 Report

Quoted By:

>>12885900
the joys of working with zinc

Anonymous

Anonymous Fri 26 Mar 2021 16:24:17 No.12886192 Report

Quoted By: >>12886222

>>12885900
Can you give me the exact details of what you did? I'm curious, and might try to recreate it, to see if our resaults match.

Anonymous

Anonymous Fri 26 Mar 2021 16:29:05 No.12886215 Report

Quoted By:

>>12885900
don't let it escape!

Anonymous

Anonymous Fri 26 Mar 2021 16:30:33 No.12886222 Report

Quoted By: >>12886254

>>12886163
>>12886192
Potassium Alum + C.I. Sulfur Black 1 + Methylchloroisothiazolinone + Methylisothiazolinone + Sodium Metasilicate

Anonymous

Anonymous Fri 26 Mar 2021 16:40:32 No.12886254 Report

Quoted By: >>12886286

>>12886222
If i were your prof, id give you an F. This is not enough info to recreate what you are doing, or to understand what you are trying to do. Did you just chuck it all in a 250 ml beaker and wait? Did you apply any heating or stirring? In what order and what amounts did you add of the materials? What is your solvent? What was the desired series of reactions? What is the desired end product, and waht do you think you ended up getting?

Anonymous

Anonymous Fri 26 Mar 2021 16:52:39 No.12886286 Report

Quoted By: >>12886324

>>12886254
Unfortunately, I hadn't logged the experiment because it was more a test and I didn't want to publish anything about it. Just wanted to see if the solution could grow black crystals, which I had suspected based on the Sulfur Black 1, but is now obviously not the case. What I remember: everything was added in equal amounts to a 250ml beaker of demin water and slowly heated to 75°c until dissolved completely into the solution.

Anonymous

Anonymous Fri 26 Mar 2021 17:01:07 No.12886324 Report

Quoted By: >>12886364

>>12886286
interesting, it could be fun to dick around with, though ill have to see if i have all of the above. One last question: equal amount by weight or by mole?
All that grew over one single night?? Damn, thats some quick crystal formation. Did you try hanging some small crystal in the middle to get one solid crystal?

Anonymous

Anonymous Fri 26 Mar 2021 17:01:14 No.12886326 Report

Quoted By: >>12886364

>>12885900
I'm guessing it creates some heavy vapor, that reacts with water in the air to deposit a metal oxide salt? Probably wicks up the solution too?

Anonymous

Anonymous Fri 26 Mar 2021 17:09:39 No.12886364 Report

Quoted By: >>12886385

>>12886324
Equal by weight and yes, grew overnight at room temperature. Should've added that I hung a potassium alum seed crystal into it. Crystal grew bigger staying in its octahedral shape.
>>12886326
>I'm guessing it creates some heavy vapor
Most certainly. If I come half a meter close to it I can already smell it. It's a very earthy smell.

Anonymous

Anonymous Fri 26 Mar 2021 17:14:40 No.12886385 Report

Quoted By: >>12886480

>>12886364
>It's a very earthy smell.
Nice, nothing like a bit of lung damage. I accidentaly formed a shitton of NO2 whilst making nitric acid a few monts back. Hurt like a bitch for a week. Oh well, fumehoods are expensive.
As long as i have a couple of chemistry anons here, if im trying to make borated candle wax, what kind of lard should i use to source the carboxylic acid? Or not even lard? What kind of carboxilyc acid is best for candlewax? Any tips?

Anonymous

Anonymous Fri 26 Mar 2021 17:31:37 No.12886458 Report

Quoted By: >>12886474

if you play music it will grow faster

I suggest Mozart

Anonymous

Anonymous Fri 26 Mar 2021 17:33:49 No.12886472 Report

Quoted By: >>12886481 >>12886492

>>12885900
lmao you're just trying to grow colored crystals, aren't you

Anonymous

Anonymous Fri 26 Mar 2021 17:34:07 No.12886474 Report

Quoted By:

>>12886458
isn't that plants?

Anonymous

Anonymous Fri 26 Mar 2021 17:35:39 No.12886480 Report

Quoted By: >>12886521

>>12886385
>I accidentaly formed a shitton of NO2 whilst making nitric acid a few monts back
This reminds me when I overheated a small solution of potassium ferrocyanide by mistake and it released hydrogen cyanide. A very stingy bitter almond-like fart that instantly alarmed me. Except a short lightheadedness nothing negative happened

Anonymous

Anonymous Fri 26 Mar 2021 17:35:55 No.12886481 Report

Quoted By: >>12886501

>>12886472
Hey, there is nothing wrong with growing coloured crystals. Though if thats OP's only aim, i would suggest getting a welding machine, or a shitton of microwave transformers, and making rubys.

Anonymous

Anonymous Fri 26 Mar 2021 17:37:35 No.12886492 Report

Quoted By: >>12886501

>>12886472
Besides mostly synthesis I love growing crystals, yeah.

Anonymous

Anonymous Fri 26 Mar 2021 17:39:52 No.12886501 Report

Quoted By: >>12886573

>>12886481
>>12886492
Yes anon, I didn't mean to disrespect the crystals. I too in fact love to grow crystals and mess with them in polarized light and whatnot. But OP made it sound like some next level grad school metaloorganic chemistry shit but the beaker looks like it has a string attached to seed the growth of a cool looking crystal.

Lets see that crystal OP. Post it!

Anonymous

Anonymous Fri 26 Mar 2021 17:43:26 No.12886521 Report

Quoted By: >>12886540 >>12886645

>>12886480
Yeah, you actually need a decent amount of that to die. Hence the largish pill people used back in the day. Still, im considering building my own fumehood. A couple of processor fans an arduino and some carbon filters can go a long way. Unfortunatly i don't know much about aerodynamics. The problem is, whenever i get any money, i have more interesting things to spend it on then protecting my health. You know? Like a pcr only costs 1000 euros or a nanopore DNA sequencer only costs 900 euros. A decent centrifuge only costs like 100 or 200 max. And i really need one of those.

Anonymous

Anonymous Fri 26 Mar 2021 17:46:33 No.12886540 Report

Quoted By: >>12886575

>>12886521
>pcr only costs 1000 euros
The heating block is made out of silver and weighs about a kilo or so. I think its a good investment, even if your DIY genetic engineering doesn't pan out you can short the silver.

What do you wanna do exactly?

Anonymous

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Anonymous Fri 26 Mar 2021 17:53:40 No.12886573 Report

Quoted By: >>12886584 >>12886604 >>12887280

>>12886501
Here it is. Looks like a regular potassium alum crystal BUT there's some interesting dots on it

Anonymous

Anonymous Fri 26 Mar 2021 17:53:50 No.12886575 Report

Quoted By: >>12886604 >>12887280

>>12886540
Well im doing my thesis on anti aging technology, but just for dicking around at home, right now i have two projects in mind. I want to systematicly delete pieces of non coding DNA in C elegans, to prove it has an important role in structure of the genome and by extention either epigenetics or gene interactions. The other projet is i want to do a bit of germline edditing in mice, wherein i make different fluorescent proteins express in different tissue.

Anonymous

Anonymous Fri 26 Mar 2021 17:54:59 No.12886584 Report

Quoted By: >>12886618

>>12886573
Damn, thats nice. Do you mind if i ask, are you doing this at a home lab, or at a uni/work lab?

Anonymous

Anonymous Fri 26 Mar 2021 17:58:12 No.12886604 Report

Quoted By: >>12886674

>>12886573
Thats a nice alum crystal anon

>>12886575
Wouldn't you need to inject C. elegans germline tissue with your DNA constructs to alter their genomes? The microscope+microinjector is not exactly PCR price tier. I guess you'd need even more complicated setup for the mouse shit.

Anonymous

Anonymous Fri 26 Mar 2021 18:01:18 No.12886618 Report

Quoted By:

>>12886584
Thank you, just a home lab. Really helps to relax after a hard day in the office and is more rewarding.

Anonymous

Anonymous Fri 26 Mar 2021 18:06:23 No.12886645 Report

Quoted By:

>>12886521
>Still, im considering building my own fumehood.
Same here, moving in about two months into a new house and this will be my first project.

Anonymous

Anonymous Fri 26 Mar 2021 18:12:30 No.12886674 Report

Quoted By: >>12886740

>>12886604
Yes, to pretty much everything you said. I have an alright setup already, including a really nice microscope, and some of the basic lab stuff. I can order any DNA product online, though im looking up how to make my own right now. I found this book "methods in enzymology" in which it is suposedly explained how one can synthesize DNA with a pcr machine, though i might have to buy some aditional stuff. Regardless, i have literally all my life to build up my lab, and i get an academic scolarship for goof grades, so i have a bit of spending money each month. You seem to know what you are talking about thogh. Any tips on what im going to need?

Anonymous

Anonymous Fri 26 Mar 2021 18:26:43 No.12886740 Report

Quoted By: >>12886863

>>12886674
Wouldn't you need to inject Cas9 + guide RNA (RNP format) to create a genomic deletion though? If you inject your PCR product with sonicated OP50 (E. coli strain normally used to feed C. elegans) carrier DNA you can create simple or complex integration arrays but I don't see how you can create deletions this way. All in all you need the microscope + injection system, a stereomicroscope to pick the progeny and then basic PCR setup to genotype the progeny for the desired modification.

What microscope do you have btw? Asking cause I like to collect stinky pond water and bait weird microorganisms like slime molds, Oomycetes and protozoa using boiled grains and then culture them at home to look under a microscope. During the week I produce recombinant proteins and set up in vitro reactions or do pulldowns for western blot and mass spec (also some other stuff as well but protein biochemistry comes naturally to me).

Anonymous

Anonymous Fri 26 Mar 2021 18:49:12 No.12886863 Report

Quoted By: >>12887241

>>12886740
Yes, i do need cas9, but I can buy cas9, and that whole setup easely enough, thats not particularly expensive. I also have a microscope, and a stereomicroscope. What i don't haev is the pcr and the injection system. I'll have enough money for both, by the end of the summer. I already have a decent bacteriology setup, though my incubator is home made and verys its temperature by 0.1 C on average, but that hasn't been a problem yet. My main problem is that i dont have official strains of C elegans or e coli. Not at home at least. And i know of no way to get my hands on it legally without pocketing shit from my uni lab.

Anonymous

Anonymous Fri 26 Mar 2021 19:54:27 No.12887241 Report

Quoted By:

>>12886863
If you can order DNA online, shouldn't you be able to order commercial strains?

Anonymous

Anonymous Fri 26 Mar 2021 20:02:54 No.12887280 Report

Quoted By: >>12888687

>>12886573
>>12886575
At first I thought you were a dumbass OP, now I realize you are a based mad scientist.
Good luck with your research, I am interested in that stuff too, maybe in grad school.

Anonymous

Anonymous Sat 27 Mar 2021 02:22:09 No.12888687 Report

Quoted By:

>>12887280
Truly based

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