/sci/ - Science & Math » Thread #12737999

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Anonymous Sun 21 Feb 18:06:12 2021 No.12737999 View Reply Original Report

Quoted By: >>12738045 >>12738070 >>12738130 >>12738176 >>12738385 >>12741584 >>12741959

Anybody bought one of these CRISPR kits yet?

https://www.addgene.org/crispr/

https://m.youtube.com/watch?v=0OTQtlHRPWc

Was wanting to get into animal genome editing

Anonymous

Anonymous Sun 21 Feb 2021 18:06:51 No.12738003 Report

Quoted By: >>12738017

Fucken' hench lad, innit?

Anonymous

Anonymous Sun 21 Feb 2021 18:11:33 No.12738017 Report

Quoted By: >>12738436

>>12738003
Also anyone know where to buy genetically modified animals in Canada?

Anonymous

Anonymous Sun 21 Feb 2021 18:20:32 No.12738045 Report

Quoted By: >>12738059 >>12738061 >>12740899

>>12737999
There was an anon claiming he'd take the treatment and pose results but that was a year ago.

Anonymous

Anonymous Sun 21 Feb 2021 18:25:39 No.12738059 Report

Quoted By:

>>12738045
Either dead or lying

Anonymous

Anonymous Sun 21 Feb 2021 18:26:36 No.12738061 Report

Quoted By:

>>12738045
What treatment? I want to modify animals, not treat anyone

Anonymous

Anonymous Sun 21 Feb 2021 18:28:32 No.12738070 Report

Quoted By: >>12738114

>>12737999
Is it possible using CRISPR to the genetics of your forum post to make your image not one that was designed for Formicidae?

Anonymous

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Anonymous Sun 21 Feb 2021 18:41:00 No.12738114 Report

Quoted By: >>12738119

>>12738070
Why? The image isn't that relevant,

Anonymous

Anonymous Sun 21 Feb 2021 18:43:10 No.12738119 Report

Quoted By: >>12738146

>>12738114
Then find an image that is relevant.

Anonymous

Anonymous Sun 21 Feb 2021 18:46:42 No.12738130 Report

Quoted By: >>12738164 >>12738269 >>12741642

>>12737999

so you have the means of animal husbandry?
then for germ line genome engineering also means of culturing oocytes/ESCs? and implanting oocytes?

The dog guy in the Netflix docu never succeded with his sperm-strategy. And never will because sperm genome is >99% heterochromatin. So to edit germ line you cannot just mix sperm and your crispr kit.

intravenous injection (soma genome engineering) won't give you good results because too few cells of the body will be recombined.

GMOs cannot be bought by private persons, only by GMO-certified institutions because those animals cannot be released.

Anonymous

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Anonymous Sun 21 Feb 2021 18:51:16 No.12738146 Report

Quoted By:

>>12738119
This one is relevant to you

Anonymous

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Anonymous Sun 21 Feb 2021 18:55:41 No.12738164 Report

Quoted By: >>12738169 >>12740645 >>12741909 >>12746260

>>12738130
>so you have the means of animal husbandry?
Yes, my mice cage in my bedroom

>then for germ line genome engineering also means of culturing oocytes/ESCs?
I have a beer fridge

>and implanting oocytes?
I can buy that on addgene or The Orion I presume?

Anonymous

Anonymous Sun 21 Feb 2021 18:57:45 No.12738169 Report

Quoted By: >>12738183 >>12738226

>>12738164
you're obviously incompetent. Before doing anything read a book or two about mouse husbandry and genetic engineering.

Anonymous

Anonymous Sun 21 Feb 2021 19:00:09 No.12738176 Report

Quoted By: >>12738274

>>12737999
Is it possible to make your gut flora capable of metabolizing micro plastics?

Anonymous

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Anonymous Sun 21 Feb 2021 19:01:11 No.12738180 Report

Quoted By: >>12738226 >>12738259

>The dog guy in the Netflix docu never succeded with his sperm-strategy. And never will because sperm genome is >99% heterochromatin. So to edit germ line you cannot just mix sperm and your crispr kit.
What else do you need to do?

>intravenous injection (soma genome engineering) won't give you good results because too few cells of the body will be recombined.
Yea I realise that, I wouldn't waste time on something that would have such a high risk with little benefit

>GMOs cannot be bought by private persons, only by GMO-certified institutions because those animals cannot be released.
How do I become a GMO-certified institution in Canada? Aren't most pets genetically modified through selective breeding? Why can't I buy a super mouse and start my own pet breed?

Anonymous

Anonymous Sun 21 Feb 2021 19:02:33 No.12738183 Report

Quoted By: >>12738313

>>12738169
Nah, think I'll just take the shortcut

Anonymous

Anonymous Sun 21 Feb 2021 19:16:23 No.12738226 Report

Quoted By: >>12740538

>>12738169
Have a (((you)))

>>12738180
Who's she?

Anonymous

Anonymous Sun 21 Feb 2021 19:26:51 No.12738259 Report

Quoted By: >>12738305 >>12741642

>>12738180
nothing, the sperm thing is a dead end. for germ line engineering one needs to engineer the oocyte.

i have no idead about those regulations. And pets are not GMOs. they are selectively bread. That is something quite different because you don't manipulate isolated dna sequences.

Anonymous

Anonymous Sun 21 Feb 2021 19:30:07 No.12738269 Report

Quoted By:

>>12738130
>And never will because sperm genome is >99% heterochromatin. So to edit germ line you cannot just mix

I think anon was talking about pronuclear injection in a oocyte

Anonymous

Anonymous Sun 21 Feb 2021 19:31:38 No.12738274 Report

Quoted By: >>12740704

>>12738176
>plastic eating bacteria
God, imagine the chaos that would ensue

Anonymous

Anonymous Sun 21 Feb 2021 19:40:02 No.12738305 Report

Quoted By: >>12738329

>>12738259
Selective breeding, manipulating isolated DNA, tomato, tomata

Anonymous

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Anonymous Sun 21 Feb 2021 19:41:16 No.12738313 Report

Quoted By:

>>12738183
Based

Anonymous

Anonymous Sun 21 Feb 2021 19:46:04 No.12738329 Report

Quoted By: >>12738398

>>12738305
Not according to regulators. Scientists don't set the laws

Anonymous

Anonymous Sun 21 Feb 2021 20:02:07 No.12738385 Report

Quoted By:

>>12737999
This might help anon:
https://icm.criver.com/QOS_FO/AASelectService.aspx

Not sure if they'll let you import it to canada

Anonymous

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Anonymous Sun 21 Feb 2021 20:07:36 No.12738398 Report

Quoted By:

>>12738329
You were saying?

https://www.loc.gov/law/help/restrictions-on-gmos/canada.php#_ftn16

Anonymous

Anonymous Sun 21 Feb 2021 20:21:55 No.12738436 Report

Quoted By:

>>12738017
Knockout mice?

https://www.cyagen.com/us/en/catalog-model-bank.html

Anonymous

Anonymous Mon 22 Feb 2021 07:10:15 No.12740538 Report

Quoted By:

>>12738226
>she

Anonymous

Anonymous Mon 22 Feb 2021 08:07:29 No.12740645 Report

Quoted By:

>>12738164

Whos that semen demon

Anonymous

Anonymous Mon 22 Feb 2021 08:38:54 No.12740704 Report

Quoted By:

>>12738274
The plastic revolution and it's consequences have been a disaster for the human race.

Anonymous

Anonymous Mon 22 Feb 2021 10:59:21 No.12740899 Report

Quoted By:

>>12738045
he has/had a blog on zeronet but I dont have a link
he was an Australian who wanted to make a super soldier type treatment.

Anonymous

Anonymous Mon 22 Feb 2021 16:06:05 No.12741584 Report

Quoted By:

>>12737999
>Anybody bought one of these CRISPR kits yet?
>https://www.addgene.org/crispr/
Do you seriously think they will sell you a CRISPR kit if you’re not a legit lab?

Anonymous

Anonymous Mon 22 Feb 2021 16:28:01 No.12741642 Report

Quoted By: >>12742945

>>12738259
The sperm thing is honestly fine it's not like sperm mediated transfer is new or anything and has been done repeatedly
>>12738130
Injecting plasmid into somatic cells can give decent results depending on what specifically you are trying to alter for example if you simply are attempting to increase the production of xxx then it's likely to work fine if you dump a shitload into some muscle or fat cells

Anonymous

Anonymous Mon 22 Feb 2021 17:59:05 No.12741909 Report

Quoted By:

>>12738164
aside from nudity i find it exceptionally hot

Anonymous

Anonymous Mon 22 Feb 2021 18:18:41 No.12741959 Report

Quoted By:

>>12737999
in awe at the size of this mouse, absolute unit

Anonymous

Anonymous Mon 22 Feb 2021 21:38:57 No.12742945 Report

Quoted By: >>12742949 >>12743566 >>12744679

>>12741642
youre talking cell culture. OP wants to create mutant mice. knock-in over-expression somatically through CRISPR in mice? This will never have any results.

in sperm maybe a point mutation or so. But not more than that I doubt it. As far as I know no even slightly sophisticated genome engineering succeded with sperm (episomal plasmids is a different story!)

Anonymous

Anonymous Mon 22 Feb 2021 21:40:13 No.12742949 Report

Quoted By:

>>12742945
*never succeded with sperm

Anonymous

Anonymous Tue 23 Feb 2021 00:37:27 No.12743566 Report

Quoted By: >>12744698

>>12742945
yeah OP should really consider working with flies or worms first, a lot simpler, unless you're doing screens or working with EthBr or anything nasty it's not even all that risky.

OP, fucking around with mice AFAIK requires approval of whatever you're doing, there's ethics boards that spend their entire day approving experiments, if you publish anything you do you could get in real trouble, even if you don't publish I dunno, maybe I drank the kool-aid but genetic experiments in mice without a discernable goal that would further research or benefit society in some way makes me a touch uncomfortable. if you worked in the field it would raise eyebrows to say the least. why not work with flies? or if you can't be sure about containment then there are plenty of invertebrate model organisms out there. sea squirts are popular these days. c. elegans are a classic for a reason.

Anonymous

Anonymous Tue 23 Feb 2021 06:56:21 No.12744679 Report

Quoted By: >>12746653

>>12742945
Ya, I'd have to agree that using crispr for that result in already developed mice wouldn't be the greatest although if he simply wanted to over express something then he could honestly just inject a shitload of naked plasmid and it'd probably give noticeable results
>in sperm maybe a point mutation or so
We can actually get fairly decent results fiddling with sperm see
https://doi.org/10.1186/1472-6750-2-5
I'm fairly sure there is an article from like 1998 somewhere that also had success

Anonymous

Anonymous Tue 23 Feb 2021 07:03:13 No.12744698 Report

Quoted By:

>>12743566
You actually don't need to go through any ethics boards at all it's just the standard in labs and if you're doing it at home there is 0 oversight you need to legally adhere to in just about every country given how rare it is that something sets up a genetics lab in their own home for a hobby.

Even i'm thinking of starting animal testing in my garage for some interesting genes to add a bit of spice to fiddling with yeast and e.coli

Anonymous

Anonymous Tue 23 Feb 2021 12:59:06 No.12745448 Report

Quoted By:

bump

Anonymous

Anonymous Tue 23 Feb 2021 17:35:36 No.12746260 Report

Quoted By:

>>12738164
My God that's hot as fuck

Anonymous

Anonymous Tue 23 Feb 2021 19:49:18 No.12746653 Report

Quoted By:

>>12744679
thanks for ref

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