https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004504
> Here, we study cell division in E. coli strains lacking both the Min system and nucleoid occlusion factor SlmA to identify new mechanisms involved in Z-ring localization. We use high-resolution quantitative fluorescence imaging to resolve nanometer-scale changes in positions of the Z-rings and cell division planes. We show that Min and SlmA double deletion cells are capable of accurately localizing their division planes in slow growth conditions. In this process, E. coli frequently positions its Z-ring initially over the nucleoid center instead of at nucleoid-free regions. We determine that during the formation of the Z-ring, the nucleoid center is occupied by the Ter macrodomain region of the chromosome. MatP, ZapB, and ZapA proteins, which have been implicated in linking the Ter macrodomain and the Z-ring [25], affect the accuracy and the precision of the Z-ring positioning relative to the nucleoid center.
>However, E. coli ?slmA ?min cells without MatP, ZapB, and ZapA are still capable of positioning their Z-rings close to the cell centers, albeit with lower precision.
I wonder if another(fourth?) midcell positioning system of Z-ring in E.coli was discovered during six years since this article was written? I have googled but I couldn't find.
If this question is pretty specific, in what places else can I ask similar questions about molecular biology?
> Here, we study cell division in E. coli strains lacking both the Min system and nucleoid occlusion factor SlmA to identify new mechanisms involved in Z-ring localization. We use high-resolution quantitative fluorescence imaging to resolve nanometer-scale changes in positions of the Z-rings and cell division planes. We show that Min and SlmA double deletion cells are capable of accurately localizing their division planes in slow growth conditions. In this process, E. coli frequently positions its Z-ring initially over the nucleoid center instead of at nucleoid-free regions. We determine that during the formation of the Z-ring, the nucleoid center is occupied by the Ter macrodomain region of the chromosome. MatP, ZapB, and ZapA proteins, which have been implicated in linking the Ter macrodomain and the Z-ring [25], affect the accuracy and the precision of the Z-ring positioning relative to the nucleoid center.
>However, E. coli ?slmA ?min cells without MatP, ZapB, and ZapA are still capable of positioning their Z-rings close to the cell centers, albeit with lower precision.
I wonder if another(fourth?) midcell positioning system of Z-ring in E.coli was discovered during six years since this article was written? I have googled but I couldn't find.
If this question is pretty specific, in what places else can I ask similar questions about molecular biology?
