/sci/ - Science & Math » Thread #12420045

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Anonymous Fri 04 Dec 20:00:10 2020 No.12420045 View Reply Original Report

Quoted By: >>12420143 >>12420208 >>12420518 >>12420849

How big of a risk is contamination anyways?

>be me
>working with grad students
>developing bioreactor
>my partner insists on using peristaltic pumps to extract and replenish media
>inefficient as shit
>we could literally rinse pic related with ethanol and use it to pump at 150x the speed
>muh sterility

I feel like if you're not being excessively reckless contamination isn't even a problem. Am I retarded?

Anonymous

Anonymous Fri 04 Dec 2020 20:34:48 No.12420127 Report

Quoted By:

Yes

Anonymous

Anonymous Fri 04 Dec 2020 20:40:59 No.12420143 Report

Quoted By:

>>12420045
>peristaltic pumps
never heard of these till now
once tried to fill a balloon like that by hand for fun. after an hour the balloon went from empty to the size of a cherry and I gave up. good luck OP

Anonymous

Anonymous Fri 04 Dec 2020 20:42:12 No.12420148 Report

Quoted By:

On a small scale like pipetting things yeah as long as you're not a dumbass you won't contaminate your media etc. but when you're pumping a large volume the chances of contamination go up exponentially, and once you've got some yeast or whatever in your media that's it you have to start over.

I'd say the time savings from using a cheaper/quicker pump that is more prone to introducing contamination is significantly less than the time youd have to invest starting over after you find bacteria goop in your precious bioreactor

Anonymous

Anonymous Fri 04 Dec 2020 21:14:03 No.12420208 Report

Quoted By:

>>12420045
There's basically no reason not to use a peristaltic pump for bioreactors. You have greater chance of avoiding biological and chemical contaminants (pump oil from mechanical pump will always leak for instance even if you don't get fungus or bacterial outbreaks). Considering you have the time to shitpost I doubt you are on such a strict time schedule that you can't use a proper pump.

Anonymous

Anonymous Fri 04 Dec 2020 23:03:21 No.12420518 Report

Quoted By:

>>12420045
OZONE is your friend.

Anonymous

Anonymous Sat 05 Dec 2020 00:58:14 No.12420849 Report

Quoted By:

>>12420045
>Am I retarded?
Absolutely yes. Those shitty fish tank pumps will inevitably leak or have mechanical parts that will get all gunked up and fail. Peristaltic pumps don't have any of those problems and all you need to do is change tubing. Use the fucking proper pumps.

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