>>12382274Wouldn't recommend it, would be at least $20k, just as a backyard thing, not registering as a small lab. If you're friendly with lab technicians you could probably get broken or second hand lab equipment. To cut costs you might want to look into the paper centrifuge and DIY clean room/glove boxes, but they are very essential.
On top of that you'll need
>water bath, with precise temperature control>mechanical pipette (I hear there's 3D print available) with autoclavable/replaceable tips>high pressure cooker to autoclave>incubator to anneal DNA>fridge to store temperature sensitive samples/chemcialsFor DNA extraction you can get a
>DNA extraction kits with all the lytic enzymes and protease and all that good stuff for about $10-40/per unit, but they come in packs of 50-300For DNA tinkering you'll need
>genome wide sequencing costing around $1000>restriction enzymes go for $300-700>custom sequences are about $0.50-0.80/bpI'm not well versed in putting nuclei into embryos but I think the entire process is mostly mechanical using microsyringes and tiny vacuum platforms to hold the embryo in place.
