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DNP - Dinitrophenol

Anonymous Thu 12 Nov 16:36:35 2020 No.12334111 View Reply Original Report

Quoted By: >>12335145

Can someone with knowledge of biochem or medicine explain something to me please. How is DNP toxic? I'm reading about it for uni, not for personal use, but everywhere it's mentioned, it just says it's extremely toxic and dangerous and whatever. But the dangerous effects they list are just overdose signs; hyperthermia, delirium, drowsiness...

I have a couple points I want to clear up for myself:
-how is it toxic? the chemical view, how does it react other than it's intended use as a decoupler?
-do some people just have a sort of reaction to it, like an allergy or a sensitivity? That would make it dangerous, but that's like saying peanuts are deadly because some people are allergic to them.
-hypothetically, if I were to get a hold of DNP pills and I took a steady dose of let's say 100mg per day (I've heard of people using more than 600mg per day), and I would NOT INCREASE the dose in any way, I would reach a steady-state concentration of it in my body. Every article or blog or book or whatever says that it accumulates with a half life of 2-3 days or something like that. That shouldn't matter if I take a low dose, I just shouldn't take more than my body can metabolise...
Thanks for any input.

Anonymous

Anonymous Thu 12 Nov 2020 17:13:04 No.12334281 Report

Quoted By: >>12334743

lmao just stop eating, fatty

Anonymous

Anonymous Thu 12 Nov 2020 19:23:36 No.12334743 Report

Quoted By: >>12334807

>>12334281
What? I said it's not for my own use, I just want to know why everyone seems to say it's so dangerous.

Anonymous

Anonymous Thu 12 Nov 2020 19:33:42 No.12334766 Report

Quoted By:

Retarded fatty says it's not for me but says he'll take 100mg a day. Just die already from it

Anonymous

Anonymous Thu 12 Nov 2020 19:39:01 No.12334780 Report

Quoted By: >>12334808 >>12335145

DNP decreases the formation of high-energy phosphate bonds in mitochondria and at the same time stimulates systemic oxygen consumption [23]. This dissociative effect is known as uncoupling of oxidative phosphorylation. Adenosine triphosphate (ATP) production is the final product of the tricarboxylic acid (Krebs) cycle in mitocondria along with CO2 and H2O. During glycolysis, there is a net production of two ATP molecules, but the majority of energy-rich phosphate bonds (38 in total) are produced during the final oxidative phosphorylation process. During this final phase, ATP synthetase converts adenosinediphosphate to ATP with the addition of an inorganic phosphate molecule. DNP interferes with the final energy production pathway by preventing the uptake of inorganic phosphate molecules into the mitochondria [24, 25]. This results in the inhibition of all energy-requiring processes and the extra-mitochondrial accumulation of inorganic phosphate [26]. DNP also acts as a chemical ionophore, stopping the final energy conversion by exporting the proton ions (H+) needed for ATP production across the mitochondrial membrane by increasing the basal leak of protons [12]. This shift in the proton electrochemical gradient then results in potential energy dissipating as heat, instead of being converted to ATP, with rapid consumption of calories [27, 28]. The heat production represents a failure in thermoregulatory homeostasis, leading to uncontrolled hyperthermia [29].

Anonymous

Anonymous Thu 12 Nov 2020 19:44:59 No.12334807 Report

Quoted By:

>>12334743
yea sure

Anonymous

Anonymous Thu 12 Nov 2020 19:45:04 No.12334808 Report

Quoted By: >>12334904 >>12335159

>>12334780
Thanks, finally an answer sort of in the right direction. However, it still doesn't answer my question. I know well how it works, the decoupling process and disabling ATP production in mitochondria. The Wiki article only assumes some high dose that completely destroys temperature homeostasis. Yes, of course cooking to death is dangerous, but it's not toxic. Boiling water is not toxic but can still kill you. As far as I've seen, DNP has no actual toxic effects, it works the same way as thermogenin in brown adipose tissue.

Anonymous

Anonymous Thu 12 Nov 2020 20:12:54 No.12334904 Report

Quoted By: >>12334953

>>12334808
It is toxic because it has deleterious effects on the body. I don't get what definition of toxicity you're applying here.

Anonymous

Anonymous Thu 12 Nov 2020 20:27:55 No.12334953 Report

Quoted By: >>12335523

>>12334904
Still no answer to how it kills you if you don't cook yourself. I will give an example why I'm so pissed that every source says the same thing. It's usually like this: "DNP is incredibly dangerous because it uncouples oxidative phosphorilation in mitochondira which leads to uncontrollable thermogenesis which can cause endogenous heat stroke."
Now, what I just said is all fine, but it means that as long as the concentration is low enough, you create more heat but your body can cope with it just fine.
Every article assumes that you'd just use a ton of it and cook yourself. That can happen and it has happened, but it means that if you're smart with it, no real problem.
Another "problem" mentioned is the long half life in the body, which is over a day. That doesn't matter since it just means that your steady-state concentration will be higher than the daily dose.

What I want to know is if there is another way that this is dangerous? From all that I've heard there's just the problem with misuse and overdosing. It's not a problem for your liver or kidneys, doesn't damage your cell membranes (other than the inner mitochondrial membrane).

Anonymous

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Anonymous Thu 12 Nov 2020 21:32:30 No.12335145 Report

Quoted By: >>12335457

>>12334111
As >>12334780 copypasted, it is mainly a decoupler, I guess it can have other toxic effect like other nitrogenated aromatic compounds, but these would be negligible compared to decoupling. In the mitochondrion, energy-rich compounds from food are used to generate H+ gradient that is later used for ATP synthesis (see chemiosmotic theory). This gradient is also used for transportation, heat generation, and other stuff. DNP shortcuts this gradient, leading just to unseful heat instead (pic related). It can be considered an analogue of those heat producing proteins (UCP), though without any regulation. In small amounts the body can compensate this, but higher doses of DNP are fatal for obvious reasons - your body cannot convert food to useful energy, but only heat - therefore the hyperthermia.
DNP used to be an unwanted side-product during picrate (explosives) syntheis - even though the workers ate normally or even more than average man, they used to be very thin and fragile due to DNP.

tl;dr: DON'T USE DNP

Anonymous

Anonymous Thu 12 Nov 2020 21:38:04 No.12335159 Report

Quoted By: >>12335418

>>12334808
>As far as I've seen, DNP has no actual toxic effects, it works the same way as thermogenin in brown adipose tissue.
*facepalm*
Thermogenin (UCP1) is under regulation retard, unlike DNP. Uncoupling isn't just about heat production, you basically lose the ATP production, you claim to know how that works, so not sure if you're trolling or just stupid.

Anonymous

Anonymous Thu 12 Nov 2020 23:31:26 No.12335418 Report

Quoted By: >>12335457 >>12335473

>>12335159
No I get it, I get that it's uncontrolled internally but that's why you control the dose. Every article ever written talks about DNP like it's botulinum or some super deadly poison that will kill you if you look at it wrong.
Yes it can be deadly if you use too much, but your mitochondria can deal with a bit of proton leakage, i'd think.

Anonymous

Anonymous Thu 12 Nov 2020 23:49:40 No.12335457 Report

Quoted By:

>>12335418
>Every article ever written talks about DNP like it's botulinum or some super deadly poison that will kill you if you look at it wrong.
Well that's bs ofc. As I wrote in >>12335145, workers were affected by it on lower doses, and despite being thin, they were capable of quite normal life. In lab we treat it like a general poison, but it definitely not as insane as dimethyl-(*insert your favourite toxic metal*) compounds.
>Yes it can be deadly if you use too much, but your mitochondria can deal with a bit of proton leakage, i'd think.
It probably depends where it strikes - I can imagine decoupling is less fatal in muscles etc., but neural network has an insane ATP consumption due to proton pumps, so the act of decoupling can be much more fatal. Just an opinium though.

Anonymous

Anonymous Thu 12 Nov 2020 23:57:53 No.12335473 Report

Quoted By:

>>12335418
How about just eat less you disgusting fat fuck
It makes you thinner by wasting your cells respiratory energy.
Why not just do meth if you want to lose weight, it would put far less oxidative stress on your cells and arteries.

Anonymous

Anonymous Fri 13 Nov 2020 00:16:05 No.12335523 Report

Quoted By:

>>12334953
The disruption of the body's normal chemical processes (i.e. decoupling of oxidative phosphorylation) is the toxicity. It's not "toxic" in very small amounts in the same way very small amounts of cyanide aren't. Toxicity doesn't mean your body has to turn green

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