>>12127848The sites directly abut the sequence forward and reverse. Now given the alignment it's not too far fetched to think the sites would have appeared by point mutations.
However , RBM is directly linked to the virulence of this strain. The sites are also directly in the same frame of RBM's ORF.
I do not say it's impossible, but it's quite fishy as the emergence of flanking sites in this very specific configuration is for me unlikely.
A RES can appear in any random 6N sequence but all three events (RBM emergence and 2 site emergence within this viral breakthrough mutation) must be compatible, that lowers your overall likelyhood.
Now I'm interested with that 4k configuration possible. What did you do to arrive to this ?
