>>12108313Because is not that simple. What you want is a concentrate of bacteria that you can use and distribute and purifying the toxins is even harder(unless you dont care, and want to risk getting nothing, then just let the bacteria go through lisis and centruge the solution). In your example clostrodium botulinum is a strict anaerobic, that means that you have to use a co2 jar that displaces all the oxygen(havent heard if you can grow them on agar by pouring it over them in the petri) then take into account that thry will require a lot of time to reach a usefull number(they will kedp conuming nutrients in this process basedou better did not fuck up the composition of the media) and then the concentration of toxin would be quite low per bacteria, not enough to hurt anyone.
After you have the concentrate you need storage. which is actually the hardest part, because if you lose product while you acumulate it, whats the point. In this case the toxin is a protein so it has to be stored in a cold environment with a specific concentration of ions and pH or the protein might denaturalize(add a buffer for safety)
The the delivery method depend on your objectives, but aerosols will force you into having a muh higher ammount of toxin, than say, dropping it into a glass of water. and after that, have in mind that the toxin in the environepment can degrade quickly if its a sunny or rainiy day.
By the time you are finished you have a lab and more skills than the average pippeting monkey, so you give up your bioterrorist ways and apply for bayer to get the big bucks.
