/sci/ - Science & Math » Thread #11990784

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Anonymous Sun 09 Aug 16:52:38 2020 No.11990784 View Reply Original Report

Quoted By: >>11990902 >>11991059 >>11993104

How do we build DNA de novo? For example if I want to make the sequence ACGTACGT from scratch how do people do this?

Anonymous

Anonymous Sun 09 Aug 2020 16:55:53 No.11990800 Report

Quoted By:

Why do you need to make DNA?
It's made from precursors in cells.
You probaly can make short sequences in a lab.

Anonymous

Anonymous Sun 09 Aug 2020 17:21:52 No.11990902 Report

Quoted By:

>>11990784
https://en.wikipedia.org/wiki/Oligonucleotide_synthesis

there is tons of literature on this that's not wikipedia that will tell you how , you can just order it from a company if you need it

Anonymous

Anonymous Sun 09 Aug 2020 18:04:38 No.11991059 Report

Quoted By:

>>11990784
there was a young man who made a machine that used light to synthesize de novo very fast. he was 'siouxicided'

Anonymous

Anonymous Sun 09 Aug 2020 18:06:34 No.11991069 Report

Quoted By: >>11991708

Do you need primers, a plasmid or you're asking how is DNA synthesized from the nucleotides?

Anonymous

Anonymous Sun 09 Aug 2020 18:06:47 No.11991071 Report

Quoted By: >>11991708

Why?

Anonymous

Anonymous Sun 09 Aug 2020 20:37:12 No.11991708 Report

Quoted By:

>>11991071
I want to make viral vectors
>>11991069
Im just curious about the process, like how big companies do it. Some basic steps would be nice

Anonymous

Anonymous Sun 09 Aug 2020 20:50:41 No.11991738 Report

Quoted By:

I'd like to put a paste of carbon, hydrogen, oxygen, nitrogen, and phosphorus atoms into some kind of 3D printer but for DNA. Computers became consumer friendly, why can't lab equipment be the same?

Anonymous

Anonymous Mon 10 Aug 2020 08:56:31 No.11993104 Report

Quoted By: >>11993107

>>11990784
*SNIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF*

Anonymous

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Anonymous Mon 10 Aug 2020 08:57:53 No.11993107 Report

Quoted By:

>>11993104
based zoomer

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