Hi all,
I've been researching methods to transform plant cells to express the GFP (or preferably eGFP). I have a degree in biochemistry, so I don't think it's an impossible dream - but after years of teaching high school, a lot of what I learned in college has slipped away.
I have decided that the Agrobacterium method makes the most sense. I'd like to treat the pollen to get full plant-wide expression, but at this point any level of expression would be cool.
I can purchase Agrobacterium online, and there are many GFP-containing plasmids I can order.
It would be awesome if I could simply order Agrobacterium that is already loaded with the GFP gene somewhere. If that isn't possible, I'm hoping to get some advice on the best way to prepare the bacteria myself; the "freeze-thaw" method seemed doable for a DIY hobbyist, but I'm not certain.
Then, there's the question of how to introduce the Agrobacterium to the plant cells to get the best results, but I'll worry about that once I get to it.
Can anyone point me in the right direction, or link me to some resources where I can learn more?