>>11709180and as a follow-up, viral transfection is not the only means of transfection. from personal experience with S2 cells, calcium phosphate transfection works even better than lipid transfection (which gets used a lot for animal cells). since mosquito cells are also insect cells it would definitely be worth trying out. there's no need for a viral transfection vector whatsoever, in fact. and what they typically do for the viral vectors is they remove the machinery for clipping the gene out of the host cell genome which prevents viral amplification (reproduction) and effectively neuters the virus. the real concern for viral vectors is that if a gene gets inserted into the wrong spot, it could cause cancer by disrupting a tumor suppressor gene, which is how certain viruses like RSV and such cause cancer. but really, if the viral vector is properly modified, it shouldn't be risky even if it is used.
but most likely, with a mosquito, nobody would bother to use a viral vector since CaP transfection would be cheaper, easier, and reliable (at least in S2 cells, which are also insect cells - I haven't seen any specific data about CaP transfection in mosquito cells).
