>>11658798>pieces of the virusHere's why it's 'pieces'
"The situation with SARS-CoV-2 is fundamentally different because this virus is assigned to an existing species that contains hundreds of known viruses predominantly isolated from humans and diverse bats. All these viruses have names derived from SARS-CoV, although only the human isolates collected during the 2002–2003 outbreak have been confirmed to cause SARS in infected individuals. Thus, the reference to SARS in all these virus names (combined with the use of specific prefixes, suffixes and/or genome sequence IDs in public databases) acknowledges the phylogenetic (rather than clinical disease-based) grouping of the respective virus with the prototypic virus in that species (SARS-CoV)."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880/Here's an example of Korea getting the closet a full sequence, but notes this isolating:
Prior to the identification of SARS-CoV-2 as the causative agent of unknown pneumonia in Wuhan, China, pan-CoV RT-PCR was being used to detect SARS-CoV-2 in Korea. The Pan-CoV RT-PCR detects all human coronaviruses and animal-derived coronaviruses.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880/Here's another article noting where the trouble starts:
These findings indicate the different viral load kinetics of SARS-coV-2 in different patients, suggesting that sampling timing and period of the disease development play an important role in real-time RT-PCR results.
And then notes:
The occurrence of false positive with one or more of the primer and probe NTC reactions is indicative of sample contamination. Importantly, the internal control should be included to help identify the specimens containing substances that may interfere with the extraction of nucleic acid and PCR amplification.
