I'd like to build a home laboratory, mainly focused on biology, with some chemistry on the side. A project I've had in mind for a few years is to breed my own strain of baking yeast. Has anyone here done anything like this?

My list so far is:
> Trinocular Brightfield + Phase Contrast Microscope (>= 1500x magnification), preferably Inverted. (likely pick up on biosurplus, but might have to buy new. Hopefully under $6k)
> Laminar Flow / Fume Hood (probably build myself to properly fit the space, though there are many cheap used ones)
> PCR Thermal Cycler (pick up on BioSurplus for <$1000)
> Gel Electrophoresis setup (build myself)
> Lab Glassware (my friend picked up hundreds of assorted pieces, used and in various states of wear, for free. I'll pick over that and then buy anything else I need)
> Temperature-controlled cabinet for samples (build myself I think)
> Bioreactor for making a ton of yeast (build myself)
> Various Reagents, restriction enzymes, etc (buy)

My plan for yeast breeding is:
1. Expose a normal sourdough starter to various selection pressures
2. Take sample from starter, estimate density of organisms using microscope
3. Dilute sample with sterile water until on average each sub-sample contains <1 cell
4. Distribute sub-samples to growth medium, grow in sample cabinet.
5. Discard samples that show no growth, or growth from multiple places / organisms, indicating more than one cell was present in the sample.
6. Carefully grow the yeast until it is numerous enough to transfer to a sterilized flour mixture for test baking. At this point also collect a sample for PCR + Gel Electrophoresis to confirm that the test hasn't been contaminated by other strains / organisms later on.
7. After testing the properties of many strains, eventually pick some to breed in the bioreactor.
8. Dry and distribute / use the best ones.