No.11453030 ViewReplyOriginalReport
first you crack 2 healthy cells and then you pour a lil bit of restriction enzymes, let it rest a lil bit then you scoop it all up, put it in your electrophoresis kit to see if you didn't fuck up, pick it up, pour it over your plasmids, then pour it all over your e.coli and voilá

delicious cloned DNA