Any enzyme mechanism expert on here? I'm studying Plasmodium falciparum phosphocholine cytidylyl transferase (PfCCT) and i'm wondering how it can connect CDP to the ChoP residues. According to the literature, ChoP acts as a nucleophile but there is no current proposed mechanism. I'm wondering whether a Lys residue can foster a phosphate hydrolisis and how it catalytic cycle would go about.
Resources:
Coatney GR, Collins WE, Warren M, Contacos PG.The Primate Malarias.Bethesda: U.S. Department of Health, Education and Welfare; 1971.
Guca, E. et al. Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis. Sci. Rep. 8, 1–13 (2018).
Sudaratana R. Krungkrai, Jerapan Krungkrai, Insights into the pyrimidine biosynthetic pathway of human malaria parasite Plasmodium falciparum as chemotherapeutic target, Asian Pacific Journal of Tropical Medicine, Volume 9, Issue 6, 2016, Pages 525-534
Gibellini, F. & Smith, T. K. The Kennedy pathway-de novo synthesis of phosphatidylethanolamine and phosphatidylcholine. IUBMB Life 62, 414–428 (2010).
Wein, S. et al. Transport and pharmacodynamics of albitiazolium, an antimalarial drug candidate. Br. J. Pharmacol. 166, 2263–2276 (2012).
Guca, E., Contet, A., Vial, H. J., Wengelnik, K. & Cerdan, R. Phosphatidylcholine and Phosphatidylethanolamine Biosynthesis Pathways in Plasmodium. A Compr. Anal. Parasite Biol. From Metab. to Drug Discov. 171–191 (2016) doi:10.1002/9783527694082.ch7.
Resources:
Coatney GR, Collins WE, Warren M, Contacos PG.The Primate Malarias.Bethesda: U.S. Department of Health, Education and Welfare; 1971.
Guca, E. et al. Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis. Sci. Rep. 8, 1–13 (2018).
Sudaratana R. Krungkrai, Jerapan Krungkrai, Insights into the pyrimidine biosynthetic pathway of human malaria parasite Plasmodium falciparum as chemotherapeutic target, Asian Pacific Journal of Tropical Medicine, Volume 9, Issue 6, 2016, Pages 525-534
Gibellini, F. & Smith, T. K. The Kennedy pathway-de novo synthesis of phosphatidylethanolamine and phosphatidylcholine. IUBMB Life 62, 414–428 (2010).
Wein, S. et al. Transport and pharmacodynamics of albitiazolium, an antimalarial drug candidate. Br. J. Pharmacol. 166, 2263–2276 (2012).
Guca, E., Contet, A., Vial, H. J., Wengelnik, K. & Cerdan, R. Phosphatidylcholine and Phosphatidylethanolamine Biosynthesis Pathways in Plasmodium. A Compr. Anal. Parasite Biol. From Metab. to Drug Discov. 171–191 (2016) doi:10.1002/9783527694082.ch7.
