No.11234304 ViewReplyOriginalReport
Bio bros tell me how fucked am i
>be me, bio undergrad senior year
>tomorrow i will perform my first experiment for my research project and thesis
>in short i will put some sample in a yeast suspension, a protein should agglutinate the yeast by binding the mannose on their plasma membrane
>had to take sample by myself from a specific fish species i personally fished this summer
>but lazy professor didn't answer phone\emails\texts for a month and this led to an incorrect sample storing
>it stayed at 4 C° for around 35 days before finally getting frozen
>paper said proteins can stay like that for a maximum of 30 days before starting to degrade
>we'll have to sample more for other analyses, but today my prof kind of implied that if the experiment tomorrow doesn't work i should give up my project, meaning they won't help me carry on another sampling (wich would cost time and some uni money)

this is irrational because even he said that sample is no good, so the experiment failing doesn't mean the reaction would't work on a fresh sample, but anyway:
Do you think a protein can still manifest its activity, considering that it's just a fairly simple chemical bond?
Do you think it will work anyway?