>>10853887>Soccer moms A,B,C,D...Z hire a lab to do genetic work. i dont know specifics about this, but im fairly certain this is illegal at this time for a myriad of reasons, the main one being commercialization of gene editing legality. If it's underground, i dont have to worry as much, there arent too many rogue scientists with this knowledge (or that need/care about money).
>minicircle DNA random aside, but minicircle DNA isnt really needed with CRISPR techniques. you can alter genomes with CRISPR plasmids which happens relatively fast, faster than clearance of the foreign vector.
>So no direct clinical application but no worries of funding? Very strange imo. You must work in a good lab.I really do, my advisor is very well established and was one of the key figures in discovering a certain family of proteins. In their mid 60s too, so been around for a while.
>so you're clearly worried about banning GM like they did in EU. Same. People get scared of this tech really easily like nuclear which I wish didn't happen.i mean, im scared of a blanket ban of anything relating to my work. It's happened before, and it absolutely did have an impact on my advisor's research area, which is why im very wary.
>Thoughts on this technique if familiar with it?Im familiar with what it's trying to accomplish, but not directly familiar with the research behind it. What I am familiar with is a current technique which, if it works well en-vivo (which, theoretically, there is no reason it shouldnt, but you never know), will solve that problem completely.
But there's another, much bigger problem, and that is any genomic integration to post-mitotic cells will never be stable, and integration into progenitor cells has extremely low (under 1%) efficiency. If you don't get it at the embryonic stage, any genomic alteration will be like putting a drop of food coloring in a swimming pool and trying to see the color change. Terrible analogy but i hope it gets the point across.
