Italy admitted that his first love was a boy, HRE. Germany is HRE, his memories were repressed after becoming a new country. And we already know that Germany is helplessly in love with Italy. Himapapa even put a warning on his website that his comic included BL and to not read if you hated that, I'm guessing because he was getting harassed by homophobic fans.
Spain/Romano is more up to interpretation I guess, if you really wanna be finicky.>Spain: Sorry to have kept you waiting! Men can now marry each other!>Spain: Hey... Roma... Umm...>Romano: Minimum 3 free meals and a nap with pasta.