>>13902987You don’t want gene edited cripple coli. You want vital fast growing coli. I suppose you could increase NAD(H) content of the cell somewhat but its not really worth it. I suggest looking into what real world manufacturers use.
You need to extract it, which I suppose is the hard part. You have to either buy the lab equipment which means you are rich enough to afford bying NAD+ or make your own equipment, which means you can get rich.