I've finally finished my analog for lithobates sylvaticus mucous. It exhibits near perfect similarity in it's cellular preservation properties to the organic counterpart. So far, a 30:70 solution with saline water seems to be the best fit. More mucous helps during the slow freeze process, but too much and the cells themselves would begin to inhibit function.

In all 7 tests I ran, there was no indication of ice crystal formation inside the cells. Minimum temperature reached was 118K. In addition to this, I've found a way of automating the refill of both the primary and auxiliary pumping station using an external tank and an electric pump, thinking about getting a second pump as a dead-switch backup incase the first somehow busts. I don't believe it to be long before I can preform this process on myself, although a continuous power supply is an issue. Solar panels, maybe?