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In Family Guy Season 10 Episode 18, when Peter attempts to perform the Who's on First routine with a puma, he says the line "I'm asking YOU who's on first", implying that he's filling Lou Costello's role in the skit, but when the puma visits him in the hospital later, it says, "Hey, I get it now. Who is the man's name", suggesting that the puma, and not Peter, was playing Costello's role.
Boy, I really hope somebody got fired for that blunder.