Nen Users in Tier 1 = 56 (at least)
A) Named characters with known abilities = 16
B) Unnamed characters with known abilities = 1
C) Named characters with unknown abilities = 13
D) Unnamed characters with unknown abilities = 26
A) Benjamin, Musse, Rihan, Shikaku, Yushohi, Vincent, Camilla, Basho, Halkenburg, Melody, Hanzo, Tuffdy, Biscuit, Kurapika, Sayird, and Kurton.
B) Silent Majority's user.
C) Balsamilco, Furykov, Coventoba, Butch, Orau, Vict, Babimyna, Theta, Salkov, Izunavi, Keeney, Belerainte, and Bill.
D) Benjamin's soldiers at 1007, 1010 and 1013. Camilla's 7 guards and 5 affiliate Hunters. The bishounen Hunter with Izunavi. The 2 Hunters with Basho. The Hunter with Melody and Keeney. Seven Hunters working for Marayam.